- ￥2600
- Proteintech
- 0.156-10 ng/mL
- KE00912
- 2026年07月06日
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- 技术资料
- 检测范围：
0.156-10 ng/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Cell lysate
- 规格：
96T
Product Information
KE00912 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human KIR2DL4/CD158d ELISA kit is to be used to detect and quantify protein levels of endogenous human KIR2DL4/CD158d. The assay recognizes human KIR2DL4/CD158d. An antibody specific for human KIR2DL4/CD158d has been pre-coated onto the microwells. The human KIR2DL4/CD158d protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human KIR2DL4/CD158d is added to detect the captured human KIR2DL4/CD158d protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human KIR2DL4/CD158d ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3805 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 90%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.28 | 0.15 | 2.75 |
| 2 | 8 | 1.29 | 0.03 | 2.40 |
| 3 | 8 | 0.63 | 0.03 | 4.00 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.04 | 0.30 | 5.97 |
| 2 | 16 | 1.22 | 0.08 | 6.65 |
| 3 | 16 | 0.63 | 0.02 | 3.17 |
Background Information
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells that regulate their responsiveness to signals received from their environment. Killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4, also known as CD158D and KIR103AS) is an unusual member of the KIRs family, which activates the cytotoxicity of NK cells. KIR2DL4 has two immunoglobulin domains and a single immunoreceptor tyrosine-based inhibition motif (ITIM) in the cytoplasmic tail with weak inhibitory potential.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KIR2DL4, CD158 antigen-like family member D, CD158D, G9P, Killer cell immunoglobulin-like receptor 2DL4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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