- ￥724
- ZYMO RESEARCH
- D5040-5
- 2026年07月03日
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- 技术资料
- 规格：
80 ml
名称：M-Desulphonation Buffer (80 ml)
货号：D5040-5
类型：分子生物学实验耗材
规格：80 ml
价格：724
说明：支持常规分子生物学实验操作。
应用：分子生物学实验应用
特点：结构稳定，操作便捷
建议：操作前请确认设备匹配。
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文献和实验Preparation of Microinjection Buffer
, it is suggested that small quantities (1 gram) should be ordered and then all of it should be prepared at once. Microinjection Buffer For 50 ml: 10 mM Tris-HCl, pH 7.5 0.5 ml of 1 M Tris-HCl, pH 7.5 (autoclaved) 0.1 mM EDTA, pH 8.0 10 microliters of 0.5 M
Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
CCMB buffer to make chemical competent cell
does not appear to be necessary Test competence (see below) Good cells should yield around 100 - 400 colonies 4. CCMB80 buffer 10 mM KOAc pH 7.0 (10 ml of a 1M stock/l) 80 mM CaCl2.2H2O (11.8 g/l) 20 mM MnCl2.4H2O (4.0 g/l) 10 mM MgCl2.6H2O (2.0 g/l) 10
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