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- 规格:
40 μg
| Specificity | GenScript MonoRab™ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit specifically recognizes DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins. |
| Host Species | Rabbit |
| Immunogen | DYKDDDDK synthetic peptide coupled to KLH |
| Conjugate | Biotin |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Clone ID | 114F12C8 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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货号T501,产品描述:HA-Tag Rabbit Polyclonal Antibody;规格 50 ul / 100 ul; 货号T503,产品描述:Anti-DYKDDDDK Polyclonal Antibody;规格 50 ul / 100 ul; 货号T504,产品描述:Myc-Tag Mouse Monoclonal Antibody;规格 50 ul / 100 ul; 货号
2000™ or stock 3.4µM MPG (3µl/ml of media described in (Morris et al. 2004)) (note 3).Pulldown (1), Antibody specific Forty-eight hours following the biotin linked sense or antisense siRNA transfection the cultures are collected and a chromatin
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