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- GenScript
- A01854
- 2026年06月29日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
200 μl
| Specificity | GenScript Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb reacts with the Fc portion of human IgG but not with the Fab portion of human IgG. This monoclonal antibody has no cross-reactivity with mouse, rat, rabbit, chicken or goat immunoglobulins. |
| Host Species | Mouse |
| Immunogen | Human IgG(H&L) |
| Conjugate | Horseradish peroxidase (HRP) |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Optimal working dilutions must be determined by the end user. | |||||||||
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| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.4), 10 mg/mL BSA, 0.01% thimerosal and 50% glycerol. |
| Concentration | Not applicable |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2b,κ |
| Clonality | Monoclonal |
| Clone ID | 50B4A9 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
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