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- GenScript
- A01853
- 2026年06月29日
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- 文献和实验
- 技术资料
- 规格：
40 μg
| Specificity | The product is specific for Pembrolizumab. Thisantibody blocks Pembrolizumab binding with human PD-1 recombinant protein(Z03370, PD 1 Fc Chimera, Human) in ELISAs. |
| Host Species | Rabbit |
| Immunogen | Pembrolizumab |
| Conjugate | Biotin |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Clone ID | 90G12 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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文献和实验Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
RUVKUN Antibody Staining Protocol revised 10/29/90
hours at RT. 13. Wash for 4-24 hours at RT in Buffer B (usually do 5 quick washes and then 2-3 hours later do 1-2 more). 14. Incubate with 2 in Buffer A, 4-24 hours, RT (1:200 FITC-coupled goat anti-rabbit antibody for lin-14
Testing Rabbit Bleeds By Elisa
antibody diluted in Blocking Buffer for 2 hr at RT. I do tripling dilutions beginning at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP
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