![Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb产品图](https://img1.dxycdn.com/p/s14/2026/0629/046/0869954561061051702.jpg)
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- GenScript
- A01827
- 2026年06月29日
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- 文献和实验
- 技术资料
- 规格：
200 μl
| Specificity | GenScript Anti-Rabbit IgG Antibody (M205) recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations. The monoclonal antibody has no cross-reactivity with mouse, rat, bovine, goat, or sheep immunoglobulins and limited cross-reactivity with human immunoglobulins. |
| Host Species | Mouse |
| Immunogen | Rabbit IgG(H&L) |
| Species Reactivity | Rabbit |
| Conjugate | Horseradish peroxidase (HRP) |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Optimal working dilutions must be determined by the end user. | |||||||||
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| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.4), containing 1% BSA, 50% glycerol and 0.01% thimerosal. |
| Concentration | Not applicable |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | M205 |
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
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