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Background Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. Overview Description : Mouse monoclonal antibody to GST tag Tested applications : WB(1:2000-1:10000) ICC/IF(1:200-1:2000) IP/Co-IP(1:100-1:500) IHC(1:200-1:5000) Formulation : Liquid,100 μg at 1mg/mL Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative. Properties Immunogen : Glutathione-S-Transferase (GST) from Schistosoma japonicum Clonality : 2B8 Isotype : Mouse IgG2a Storage instruction Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C
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备注2：Anti-GST Tag Mouse Monoclonal Antibody：
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文献和实验应用。用GST融合表达系统表达外源基因时，需要对融合表达的产物进行检测和纯化，这里面就包括了GST标签抗体的应用，如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源，比如小鼠源的单克隆抗体还是兔源的多克隆抗体，一般来说，单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6]（货号#E022040）是来源于小鼠的单克隆抗体，克隆号为GST.B6，免疫原为GST多肽 。
Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation
of therapeutic monoclonal antibodies in the fields of transplantation and autoimmunity, and discuss the salient features of antibodies presently used in the clinic-notably anti-T-cell and anticytokine antibodies. The discussion will also focus on the unique
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
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