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文献和实验Isolation of Actin and Myosin Filaments
LEVEL III Materials Relaxing Solution 0.1 M KCl 0.001 M MgCl 5 mM ATP 0.016 M NaH PO Na HPO Adjust pH to 7.3 0.05 M Sodium phosphate buffer, pH
Preparation of Conventional Actin from Skeletal Muscle
) and 2 mM MgCl2 , (0.2 ml of 1 M MgCl2 per 100 ml). 6. Stir at room temp for 15 min then on ice for 15 min, which polymerizes the actin. 7. While stirring, make 0.8 M in KCl (23.4 ml of 4 M KCl per 100 ml), which dissociates tropomyosin. Stir
Isolation of Actin and Myosin Filaments
Isolation of Actin and Myosin Filaments LEVEL III Materials Relaxing Solution 0.1 M KCl 0.001 M MgCl 5 mM ATP 0.016 M NaH PO Na HPO
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