- 询价
- Polysciences
- 25376-500
- 美国
- 2026年01月17日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
东莞汉诺
- 规格：
500 molds
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文献和实验Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
(not Tris base). The pH is about 5. Don't adjust the pH. For yolk sac/placenta/tail/toes 2. Add 40 µl (100 µl for tail/toe) 25 mM NaOH / 0.2 mM EDTA. 3. Place at 95°C for 20 minutes (in a thermal cycler) 4. Add 40 µl (100 µl for tail/toe) 40 mM Tris
Pad Preparation 1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of agarose pads please let us know, bruno) 2. Apply 2mL to a 24x60 mm coverslip.
the selective functionalization of the 4?amino group of the cytosine base at pH 7 or the 2?amino group of the guanine base at pH 9.4 or at pH 7.4 in the presence of NiCl2 . In particular, the functionality?transfer reaction (FTR) under the alkaline conditions
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