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- 湖北武汉
- AK02495
- 2026年10月14日
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- 文献和实验
- 技术资料
- 保存条件：
It can be stored for 12 months at temperatures ranging from -20°C to -80°C. It is recommended to allocate optimal storage conditions for the protein. Avoid repeated freeze-thaw cycles.
- 保质期：
12个月
- 英文名：
CD254; ODF; OPGL; RANKL; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11
- 库存：
99
- 供应商：
湖北安可诺生物工程有限公司
- 规格：
20ug
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文献和实验Human FastTrack mRNA Isolation
temperature. 5.Resuspend oligo(dT) in 10ml Low Salt Wash Buffer by vortexing. 6.Centrifuge at 3,000 x g for 5 minutes at room temperature. 7.Repeat Low Salt Wash Buffer wash two more times. 8.Resuspend oligo(dT) in 800µl Low Salt Wash Buffer using a 1
This protocol is based on enrichment from 1 x 107 leucocytes (MNC). It is scalable from 1 x 107 –2 x 109 cells. Keep the cells and buffers cold (2–8°C) during the whole process. 1) Transfer 100 μl (1 x 107 ) leucocytes in Buffer
Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
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