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- 文献和实验
- 技术资料
- 保存条件:
It can be stored for 12 months at temperatures ranging from -20°C to -80°C. It is recommended to allocate optimal storage conditions for the protein. Avoid repeated freeze-thaw cycles.
- 保质期:
12个月
- 英文名:
Complement C2,C3/C5 convertase
- 库存:
99
- 供应商:
湖北安可诺生物工程有限公司
- 规格:
20ug
产品概述
重组人补体组分 C2 蛋白,由大肠杆菌表达,带有N 端 His 标签(N-His)。
产品参数
| 英文名称 | Recombinant Human Complement Component C2 Protein |
| 物种来源 | 人 |
| 表达宿主 | 大肠杆菌 |
| 表达区段 | 第 21–752 位氨基酸(aa) |
| 纯度 | 90% |
| 分子量 | 103 kDa |
| 标签 | N 端 His 标签(N-His) |
| 缓冲液 | 磷酸盐缓冲盐水(PBS) |
| 内毒素 | <1.0EU/μg |
| UniProt 编号 | P06681 |
| 别名(标准名称/缩写) | Complement C2,C3/C5 convertase |
复溶方法
1. 开盖前先离心,使样品集中于管底。
2. 用无菌蒸馏水复溶至 0.1–0.5 mg/mL,避免涡旋振荡或剧烈吹打。
3. 长期保存时,建议加入载体蛋白或稳定剂,例如 0.1% 牛血清白蛋白(BSA)、5% 人血清白蛋白(HSA)、10% 胎牛血清(FBS)或 5% 海藻糖。
4. 将复溶后的蛋白溶液分装保存,尽量减少冻融次数。
保存条件
在 −80~−20 ℃ 条件下可保存 12 个月。建议选择适宜的保存条件,并避免反复冻融。
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文献和实验Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Human complement component C2 is a critical factor of the classical complement pathway. Here we provide a method for the production of recombinant human C2 (rhC2) protein for research purposes. The human complement component C2 (hC
Purification and Characterization of Human and Mouse Complement C3
Complement component C3 is the most abundant complement protein in plasma, central to all three complement activation pathways and essential to complement amplification. Thus, it is one of the most extensively studied complement proteins
The classical complement pathway (CCP) activation is a multimolecular complex, composed of three subcomponents namely C1q, C1r, and C1s. C1q is the recognition subunit of this complex and its binding to the specific targets leads
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