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- 2026年06月18日
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50 μL/100 μL/200 μL
| 规格： | 50 μL | 产品价格： | ￥900.0 |
|---|---|---|---|
| 规格： | 100 μL | 产品价格： | ￥1500.0 |
| 规格： | 200 μL | 产品价格： | ￥2450.0 |
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
plastic cup--should be full of ice).2.Precut 3MM filter paper to size (10 cm X 7.5 cm)--Need 2.Cut nitrocellulose (handle with gloves!)to 8.5 cm X 6 cm.3.When gel is finished,recover it from the apparatus and cut off wells.You will need a glass dish
antibody. Incubate at room temp, 60 minutes or overnight at 4C. Pour off. 7. Wash 4 X 10 minutes in PBS-T (25 ml to 50 ml per wash), shaking. 8. During last wash, dilute secondary antibody (goat anti-rabbit HRP at 1:2500 i.e. 10 ul antibody in 25 ml BSA
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