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5μg/10μg/20μg/50μg/100μg/200μg/500μg
| 规格: | 5μg | 产品价格: | ¥235.0 |
|---|---|---|---|
| 规格: | 10μg | 产品价格: | ¥347.0 |
| 规格: | 20μg | 产品价格: | ¥574.0 |
| 规格: | 50μg | 产品价格: | ¥1132.0 |
| 规格: | 100μg | 产品价格: | ¥1901.0 |
| 规格: | 200μg | 产品价格: | ¥3350.0 |
| 规格: | 500μg | 产品价格: | ¥7068.0 |
Product Introduction
Bioactivity
| 名称 | Mannan Binding Lectin/MBL2 Protein, Human, Recombinant |
| 描述 | Mannan Binding Lectin/MBL2 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 24 kDa and the accession number is P11226. |
| 存储条件 | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
| 关键字 | MBP-1 | MBP 1 | COLEC-1 | COLEC 1 |
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文献和实验Challenges and Opportunities in Fractionation of Recombinant Human Mannan-Binding Lectin
Mannan-binding lectin is an oligomeric protein of the innate immune system. Both natural MBL as well as recombinant protein is a heterogeneous composition of oligomers containing from two up to at least eight structural units. Recombinant
Mannan-binding lectin (MBL) is a soluble pattern recognition molecule of the innate immune system. It is found in plasma in complex with MBL-associated serine proteases (MASPs). When MBL recognizes foreign, e.g., the surface
X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏
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