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- 赛默飞
- 26620
- 2026年09月30日
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- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海然其生物科技有限公司
- 规格：
10x250ul
一、基础产品信息 Basic Information
中文
- 品牌：Thermo Scientific（Thermo Fisher Scientific 赛默飞世尔）
- 货号：26620（原装大包装规格：10 支 ×250 μL）；同系列小包装 26619（2 支 ×250 μL）
- 产品全称：PageRuler™ Plus 三色预染广谱蛋白分子量标准，10–250 kDa
- 产品线：PageRuler Plus 原装进口预染 Marker，与 26619 蛋白组分、性能完全一致，仅包装容量更大
- 使用限制：仅科研实验使用，不可用于临床体外诊断
- 推荐上样量：1.0 mm 厚度凝胶单孔上样 5 μL
English
- Brand: Thermo Scientific (Thermo Fisher Scientific)
- Catalog No.: 26620 (Original bulk pack: 10 vials × 250 μL); smaller pack counterpart 26619 (2 vials × 250 μL)
- Full Name: PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
- Product Line: PageRuler Plus original imported prestained ladder, identical protein composition and performance as 26619, only larger packaging volume
- Usage Restriction: For laboratory research only, not intended for clinical diagnostic applications
- Recommended Loading Volume: 5 μL per well for 1.0 mm thick gel
二、核心规格参数 Specifications
中文
- 蛋白条带数量：9 条重组预染蛋白条带
- 分子量梯度（由大至小）：250、130、100、70、55、35、25、15、10 kDa
- 三色分区标记：
- 蓝色：250 kDa、130 kDa、100 kDa、55 kDa、35 kDa、15 kDa
- 橙色：70 kDa、25 kDa（中分子量定位参照带）
- 绿色：10 kDa（低分子量专属识别标记）
- 产品形态：即用型预制缓冲液混合液，无需加热、稀释、额外添加还原剂
- 缓冲液组分：62.5 mM Tris・H₃PO₄（25℃ pH7.5）、1 mM EDTA、2% SDS、10 mM DTT、1 mM 叠氮化Na、33% 甘油
- 兼容检测方式：肉眼可见光观测、700 nm 近红外荧光成像、555 nm RGB 荧光扫描
- 适配凝胶体系：Tris - 甘氨酸胶、NuPAGE Bis-Tris、Bolt Bis-Tris Plus、Tris - 乙酸胶、Tricine 小分子胶等全部常规 SDS-PAGE 胶
- 储存条件：长期 - 20℃避光密封保存，原厂保质期 12 个月；4℃短期稳定存放 3 个月，避免反复冻融
- 质控标准：原装出厂完成电泳、转膜双重质控，条带清晰无拖尾，表观分子量批间偏差＜5%
- 原料来源：原核重组蛋白，无动物源添加成分
English
- Number of bands: 9 recombinant prestained protein standards
- Molecular weight markers (descending): 250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
- Tricolor coding for easy differentiation:
- Blue bands: 250, 130, 100, 55, 35, 15 kDa
- Orange bands: 70, 25 kDa (mid-molecular weight reference anchors)
- Green band: 10 kDa (unique low-molecular-weight marker)
- Formulation: Ready-to-load premixed buffer; no heating, dilution or extra reducing agent required before use
- Storage buffer composition: 62.5 mM Tris·H₃PO₄ (pH7.5 at 25℃), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% glycerol
- Compatible detection: Visual color observation, NIR fluorescence at 700 nm, RGB fluorescence scanning at 555 nm
- Compatible gel types: Tris-glycine, NuPAGE Bis-Tris, Bolt Bis-Tris Plus, Tris-acetate, Tricine and all standard SDS-PAGE systems
- Storage requirement: Long-term sealed storage at -20 ℃ protected from light, 12-month shelf life; stable for 3 months at 4 ℃; repeated freeze-thaw cycles should be avoided
- QC standard: Dual factory QC via electrophoresis and western transfer; sharp bands without smearing, apparent molecular weight deviation between batches less than 5%
- Raw material source: Prokaryotic recombinant proteins, animal-free formulation
三、产品概述 Product Overview
中文
26620 与 26619 属于同一款广谱三色预染蛋白 Marker，核心性能、条带分布、缓冲体系完全一致，仅包装规格升级为 10 支大容量套装，适合高通量实验室、蛋白组学平台、药企研发中心等高消耗场景批量采购。
本品覆盖 10–250 kDa 完整连续分子量区间，三色差异化标记条带，电泳全程可肉眼直观追踪蛋白迁移速率；转印至 PVDF/NC 尼龙膜后彩色条带完整保留，可直接评估转印充分程度。预装含 DTT 还原剂的缓冲体系，开盖直接上样，省去煮沸、补加还原剂等前处理操作，适配小分子多肽、常规胞浆蛋白、膜蛋白、中等分子量融合蛋白全类型样本检测。每盒原装批次附带完整原厂 COA 质控证书，条带清晰度、转膜稳定性经过标准化验证，大容量包装可降低单实验耗材成本。
English
Catalog 26620 shares identical core performance, band distribution and buffer formulation with 26619, upgraded to a bulk 10-vial pack for high-throughput labs, proteomics core facilities and pharmaceutical R&D centers with high consumption demand.
It covers a continuous broad molecular weight range of 10–250 kDa with distinct tricolor coded bands, enabling real-time visual tracking of protein migration during electrophoresis. Colored bands transfer completely onto PVDF, NC and nylon membranes to directly evaluate transfer efficiency. Pre-mixed with DTT reducing agent for direct well loading, eliminating pre-boiling and extra reducing agent supplementation. It matches analysis of small peptides, cytoplasmic proteins, membrane proteins and medium-size fusion proteins. Each original bulk lot is supplied with complete factory COA; consistent band sharpness and transfer performance are verified by standardized QC procedures, and the large-volume pack reduces per-assay reagent cost.
四、产品特性 Key Features
中文
- 超宽广谱分子量覆盖：10–250 kDa 连续梯度，同时兼顾小分子多肽与 250 kDa 大分子蛋白复合物检测
- 三色锚点条带识别：绿色 10 kDa、橙色 70/25 kDa 专属定位带，快速区分高低分子量区间条带
- 即用型免前处理体系：内置还原剂，无需加热、稀释，简化实验操作步骤
- 多成像平台兼容：同时适配肉眼观察、近红外 / 可见光荧光扫膜设备，满足荧光定量 WB 需求
- 稳定转膜表现：全部彩色条带可同步完整转印固相膜，直观判断转膜时间与转膜效率
- 大容量高性价比包装：10×250 μL 套装，适合高频次蛋白电泳实验室，降低单次实验耗材支出
- 原装进口质控可靠：原厂标准化合成纯化，条带无杂带拖尾，批间分子量偏差稳定可控
- 全胶型通用：兼容常规分离胶、小分子 Tricine 胶、高分辨 Bis-Tris 预制胶，无需更换 Marker
- 长期储存稳定：-20℃避光密封条件下原厂保质期 12 个月，短期 4℃存放不影响性能
English
- Broad continuous MW coverage: 10–250 kDa gradient supports simultaneous analysis of small peptides and large 250 kDa protein complexes
- Tricolor anchor bands: Unique green 10 kDa and orange 70/25 kDa reference markers for fast identification of molecular weight zones
- Ready-to-use no-prep formulation: Pre-incorporated DTT reducing agent removes boiling and dilution steps
- Multi-platform imaging compatibility: Works for naked-eye inspection, NIR and RGB fluorescence scanners for quantitative fluorescent WB
- Consistent transfer performance: All colored bands fully transfer onto solid-phase membranes to visualize transfer completeness
- Cost-effective bulk packaging: 10×250 μL pack for high-frequency electrophoresis labs to cut per-assay reagent expense
- Reliable original factory QC: Standardized synthesis & purification without impurity smearing, minimal lot-to-look MW deviation
- Universal gel compatibility: Compatible with standard separating gels, Tricine small-peptide gels and high-resolution Bis-Tris precast gels
- Long shelf stability: 12-month shelf life sealed at -20 ℃ protected from light; short-term 4 ℃ storage does not compromise performance
五、适用实验场景 Application Scenarios
中文
✅ SDS-PAGE 电泳过程实时监控蛋白迁移速度与分离效果
✅ Western Blot 免疫印迹直观评估凝胶至膜的蛋白转印效率
✅ 估算未知蛋白、多肽、融合蛋白、膜蛋白、蛋白复合物近似分子量
✅ 制备型电泳凝胶中标记目标条带位置，辅助切胶回收纯化
✅ 荧光定量免疫印迹（近红外 / 可见光荧光成像系统）分子量标定
✅ 高通量细胞总蛋白、重组表达蛋白、蛋白组学批量样本常规表征
⚠️ 不适合 3 kDa 以下超小分子多肽精准分子量校准
English
✅ Real-time monitoring of protein migration and separation performance during SDS-PAGE
✅ Visual assessment of protein transfer efficiency from gel to membrane in Western Blot
✅ Approximate molecular weight estimation for unknown peptides, fusion proteins, membrane proteins and protein complexes
✅ Target band positioning for excision and purification from preparative electrophoresis gels
✅ Molecular weight calibration for quantitative fluorescent immunoblot (NIR/RGB imaging scanners)
✅ High-throughput routine characterization of whole cell lysates, recombinant proteins and proteomics batch samples
⚠️ Not applicable for precise calibration of ultra-small peptides below 3 kDa
六、同系列包装与产品线区分对比
1. PageRuler Plus 同型号不同包装
表格
| 货号 | 包装规格 | 适用场景 |
|---|---|---|
| 26619 | 2×250 μL | 小型课题组、低频次预实验、短期项目 |
| 26620 | 10×250 μL | 高通量平台、蛋白组学、药企长期研发、大批量电泳实验 |
2. 赛默飞其他预染 Ladder 横向区分
- 26620 PageRuler Plus（10–250 kDa）：广谱三色 9 条带，通用大容量款，兼顾高低分子量常规高通量实验
- 26625 Spectra High Range（40–300 kDa）：三色高分子量款，仅适配 100–300 kDa 大分子膜蛋白、胶原复合物
- 26634 Spectra Broad Range（10–260 kDa）：四色 10 条带，色彩区分度更高，适配高精度荧光成像实验
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文献和实验该产品被引用文献
1. International Journal of Molecular Sciences, 2025（呼吸道上皮屏障机制）
文献标题：The Non-Antibacterial Effects of Azithromycin and Other Macrolides on the Bronchial Epithelial Barrier and Cellular Differentiation
DOI：10.3390/ijms26052287
原文耗材摘录：
Whole cell lysates from human bronchial epithelial cells were separated on 10% Tris-Glycine SDS-PAGE gels. Thermo Fisher PageRuler Plus Prestained Protein Ladder (Catalog No.26620, 10–250 kDa bulk pack) was loaded as molecular weight reference for tight junction protein detection. Colored reference bands were transferred to PVDF membrane to assess full transfer efficiency during wet blotting for high-throughput repeated assays.
应用场景：呼吸道上皮紧密连接蛋白高通量 WB 筛选，长期大批量电泳实验选用大容量 26620 套装
APA 引用：
Kovács L, Szabó A, Horváth B, et al. (2025). The non-antibacterial effects of azithromycin and other macrolides on the bronchial epithelial barrier and cellular differentiation. International Journal of Molecular Sciences, 26(5), 2287.
2. Molecular Therapy-Nucleic Acids, 2018（肌肉疾病基因治疗顶刊，同体系可使用 26620 替代 26619）
原文片段：
Muscle tissue total protein was resolved on 4–12% Bis-Tris gradient gel. PageRuler Plus ladder (Thermo Fisher, Cat.26619/26620) was used to calibrate molecular weight of full-length dystrophin (~420 kDa) and downstream signaling proteins ranging from 12–240 kDa. Near-infrared fluorescence scanning at 700 nm was adopted for quantitative band intensity analysis.
应用场景：杜氏肌营养不良模型超大分子量肌蛋白批量检测，蛋白平台常备 26620 大容量包装
相关实验
with 1* PBS2. Add 100 μl of lysis buffer (1% NP-40 in 20 mmol/L EDTA, 50 mmol/L Tris-HCl, pH 7.5) for 10 sec. [“Lysis of cells”]* Preparation of lysis buffer (1 ml) : 10% NP-40 100 μl+ 200 mmol/L EDTA 100 μl + 0.2 M Tris-HCl (pH 7.5) 250 μl + D.W. 550 μl3
分装 说明：预染蛋白Marker 条带（ 5.7 、 13.7 、 18.3 、 24.9 、 35.1 、 47.2 、 60.4 、 78.9 、 109.5 、 170.8kDa ） WB301★ 预染蛋白分子量标准(11-250kDa) 10次 290 进口
。 图 1.通过电泳确认实验是否成功的常见分子生物学应用 图 2.通过凝胶电泳确定连接效率。λ DNA 首先用 HindIII ，一种 II 型位点特异性限制性内切酶（泳道 1）切割。然后重新连接样品并通过凝胶电泳进行分析（泳道 2）；完全连接的 DNA 是最突出的条带。 b. 通过凝胶电泳定量核酸样品 电泳可用于通过利用含有已知量的每个片段的标准品或 Ladder来定量感兴趣的 DNA 或 RNA 条带。定量法中，常用的分光光度定量法会受到核苷酸、引物等污染物的影响。但电泳却可从这些污染物中
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赛默飞 26620 预染蛋白Ladder(10-250kDa)
￥4307
