Biosharp/白鲨 BS-UFC-150-1000 15

蛋白浓缩，超滤离心管选择指南

。 3.解决两大行业痛点：堵膜+内毒素 堵膜怎么办？ 赛米科超滤管采用了低蛋白结合PES膜，配合优化的支撑结构，显著减少浓差极化导致的膜堵塞和蛋白质吸附。若样品较脏（如细胞裂解液），建议离心前先过0.45μm滤膜，或采用“阶梯离心”（低速预离心）模式。 内毒素影响细胞实验？ 所有赛米科超滤离心管均经过无内毒素工艺控制，内毒素水平＜0.25 EU/mL，可直接用于原代细胞培养、CAR-T细胞构建等敏感实验。 应用场景举例：你的实验该用哪一支？ 场景A：从杂交瘤细胞上清中浓缩IgG抗体（分子量~150kDa

Preparation of NeoR Murine Embryonic Fibroblasts

at 1000 RPM for 5 minutes at 10oC. Resuspend the pellet in MEF media and place the cells in a T-150 flask. Let the cells grow overnight in the incubator. Leave the flask undisturbed for 24 hours. After this time, change the medium to remove any cellular

Preparation of NeoR Murine Embryonic Fibroblasts

of cellular debris to settle out over a period of 2 minutes. Remove the supernatant into a fresh tube, bring the volume up to 50ml with MEF media and spin at 1000 RPM for 5 minutes at 10o C. Resuspend the pellet in MEF media and place the cells in a T-150