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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Chromodomain-helicase-DNA-binding protein 3
- 亚型:
Mouse IgG
- 形态:
液体
- 保存条件:
-20℃/1year
- 克隆性:
Monoclonal
- 标记物:
non-conjugated
- 适应物种:
Human,Mouse
- 保质期:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 抗原来源:
Mi2-α Monoclonal Antibody detects endogenous levels of Mi2-α protein.
- 目录编号:
WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
- 级别:
Affinity purification纯化
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Mouse
- 应用范围:
WB,IHC,IF,Flow Cyt,ELISA
- 浓度:
1mg/ml
- 靶点:
CHD3
- 抗体英文名:
Mi2-α 鼠单抗
- 抗体名:
CHD3;Chromodomain-helicase-DNA-binding protein 3;CHD-3;ATP-dependent helicase CHD3;Mi-2 autoantigen 240 kDa protein;Mi2-alpha;Zinc finger helicase;hZFH
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1100.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1880.0 |
我们的优势
•国产平替产品:性价比极高
•全流程质控:原料溯源,每批都做WB/IHC/IF/ELISA验证
•亲和纯化:去除非特异性IgG,降低背景、杂带。
•批间一致性:内控标准严于国标,尽量保证批间差小。
•技术支持:博士团队、优化稀释比、修复条件、解决背景高/条带弱。
•抗体条带:清晰、杂带少、背景干净,稳定性好。
•组织染色:特异性强、定位准,复现性不错。
•国内现货:发货快。
•和多家三甲医院合作:组织样本经验足。
产品信息
| 产品名称 | Mi2-α Monoclonal Antibody WB,IHC,IF,Flow Cyt,ELISA |
| 产品货号 | JK16128 |
| 基因名称 | CHD3 |
| 其他名称 | CHD3;Chromodomain-helicase-DNA-binding protein 3;CHD-3;ATP-dependent helicase CHD3;Mi-2 autoantigen 240 kDa protein;Mi2-alpha;Zinc finger helicase;hZFH |
| 宿主种属 | Mouse |
| 应用范围 | WB,IHC,IF,Flow Cyt,ELISA |
| 反应种属 | Human,Mouse |
| 克隆性 | Monoclonal |
| 特异性 | Mi2-α Monoclonal Antibody detects endogenous levels of Mi2-α protein. |
| 同种型 | Mouse IgG |
| 保存液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| 纯化方式 | Affinity purification |
| 保存条件 | -20℃ |
| 稀释比例 | WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications. |
| 宿主种属 | Mouse |
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文献和实验This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
对照组;d. 细胞定位;e. 组织专一性。例如,潜在胰脏癌标记物 Glypican-1 在许多人类恶性肿瘤表达中上调。GTX104557 Glypican-1 抗体使用多种细胞进行正交策略验证(如下图),且该抗体已通过 WB、ICC/IF、IHC-P、FACS、IP 和 ELISA 的全面验证。 另一个例子,胰脏癌标记物 KRAS。当 KRAS 基因突变,细胞分裂将不受控制,形成肿瘤。其中 KRAS G12D 和 G12V 突变占这些变化的 75% 成为研究的热点。在市面上有许多 KRAS G12











