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- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 英文名:
HTNV Nucleoprotein Recombinant Protein (N-GST & C-His)
- 库存:
999
- 供应商:
abinScience
- 规格:
50ug/100ug/1mg

| Product Name | HTNV Nucleoprotein Recombinant Protein (N-GST & C-His) |
|---|---|
| Catalog No. | VK646072 |
| Species | Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) |
| Expression System | E. coli |
| Predicted Molecular Weight | 54.81 kDa |
| Tested Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Form | Lyophilized |
| Endotoxin Level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Storage Buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| UniProt ID | P05133 |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions.
As the catalog reagent brand under AtaGenix Biotech (Wuhan, China), abinScience benefits from AtaGenix’s extensive expertise in antibody development and protein engineering. With a 3,000 m² laboratory facility and a comprehensive technology platform, abinScience offers over 35,000 products including antibodies, recombinant proteins, ELISA kits, and more.
Key advantages:
- 35,000+ validated research reagents
- Rigorous QC with lot-to-lot consistency
- Fast global shipping
- Dedicated technical support
Learn more: www.abinscience.cn
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文献和实验Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
总蛋白(包括质膜和细胞器膜蛋白),但费时费力。目前,有一种最新型有效的膜蛋白提取工具,并具有高纯度、高特异性、高载量温和提取的优点。是基于1980年科学家在牛眼视网膜中发现的一种命名为Rho1D4的氨基酸序列以及其对应的抗体来作为亲和标签纯化膜蛋白取得了非常好的效果,将Rho1D4抗体链接在琼脂糖或磁珠上,用于亲和层析带有Rho1D4标签的膜蛋白,能近乎完美的解决这些研究难题。1) 什么是Rho1D4?Rho1D4是指在牛眼视网膜细胞内的牛视紫红质C端的最后九位氨基酸。Rho1D4得名于与该序列
科研人必看:多肽、蛋白质、重组蛋白分不清? 应用场景有哪些?定制如何选?
动物细胞表达:最接近天然状态的表达平台,提供完整的人源化翻译后修饰 (如糖基化、磷酸化),适合重组抗体、细胞因子等需要复杂修饰的蛋白。 昆虫细胞表达:基因承载容量大,支持大分子量蛋白和激酶的表达。 『多种融合标签』 标签的选择直接影响纯化效率和下游应用,MCE 支持多种融合标签,可根据实验需求灵活搭配。 常规纯化标签:His 标签 (镍柱纯化,分子量小)、GST 标签 (Glutathione 亲和纯化,兼具促溶作用)、Fc 标签 (Protein A/G 亲和纯化,适合免疫相关实验)、Flag 标签
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