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- 文献和实验
- 技术资料
- 抗体名:
His 标签单克隆抗体(生物素标记,鼠源)
- 抗体英文名:
THE His Tag Antibody, Biotin, mAb, Mouse
- 靶点:
His 标签
- 浓度:
0.5mg/mL(常规浓度,以官方说明书为准)
- 应用范围:
ELISA、Western Blot、免疫沉淀 (IP)
- 宿主:
小鼠(Mouse)
- 适应物种:
通用
- 保质期:
- 20℃可稳定保存 半 年
- 抗原来源:
His 标签融合蛋白
- 级别:
科研级
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 标记物:
生物素(Biotin)
- 克隆性:
单克隆(Monoclonal)
- 保存条件:
-20℃,可稳定保存 半 年
- 形态:
液体(Liquid)
- 亚型:
Mouse IgG
- 免疫原:
His 标签融合蛋白
- 规格:
100μg
| Specificity | We His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide (HHHHHH) coupled to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 6G2A9 |
| Target Background | We His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column.The antibody is suitable for detecting the expression level of His in fusion proteins. |
| Synonyms | His Tag Antibody [Biotin], mAb, Mouse; |
His 标签抗体、His Tag 抗体、生物素标记抗体、鼠源抗体、单克隆抗体、抗 His 标签抗体、标签抗体、WB 抗体、IHC 抗体、IF 抗体、ELISA 抗体
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
应用。用GST融合表达系统表达外源基因时,需要对融合表达的产物进行检测和纯化,这里面就包括了GST标签抗体的应用,如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源,比如小鼠源的单克隆抗体还是兔源的多克隆抗体,一般来说,单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6](货号#E022040)是来源于小鼠的单克隆抗体,克隆号为GST.B6,免疫原为GST多肽 。
。我们早已熟知biotin和streptavidin之间能够高度特异的结合,这种特异性结合早已广泛用于免疫或者非放标记反应中。Novagen模拟Biotin设计了一个8个氨基酸的小标签Strep•Tag II,正好能特异的结合到streptavidin上,又再改造优化streptavidin与小标签的结合位点,使之与Strep•Tag II融合标签的结合力超出普通streptavidin约100倍,称为Strep•Tactin蛋白。当表达产物经过Strep•Tactin亲和纯化柱时,融合表达Strep
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