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- 文献和实验
- 技术资料
- 免疫原:
详询
- 亚型:
IgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, rat
- 保质期:
详询
- 抗原来源:
详询
- 目录编号:
详询
- 级别:
科研级
- 库存:
现货
- 供应商:
上海艾科斯生物科技有限公司
- 宿主:
Rabbit / IgG
- 应用范围:
WB, IHC, ELISA
- 浓度:
400 ug/ml
- 靶点:
Complement factor B
- 抗体英文名:
Complement factor B
- 抗体名:
Complement factor B
- 规格:
50 μL/100 μL/150 μL
【产品概述】
本产品为Complement factor B Polyclonal antibody(货号:10170-1-AP),由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体,可识别human, rat来源的靶蛋白,经测试适用于WB, IHC, ELISA等实验应用。产品形式为Liquid,偶联物类型为Unconjugated。
【背景介绍】
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.
【产品特点】
本抗体采用Antigen affinity purification方式纯化,确保了高特异性和批次间一致性。观测分子量约为93-100 kDa, 60-69 kDa。推荐稀释比:WB: WB : 1:5000-1:50000;IHC: IHC : 1:200-1:800。Western Blot检测阳性对照组织/细胞为human plasma, human blood, rat plasma。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.,储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程,确保研究人员获得可重复且可靠的实验结果。
【产品信息】
GenBank蛋白编号:BC007990;基因名称:Complement factor B;Gene ID (NCBI):629;UNIPROT ID:P00751;RRID:AB_2082384;别名包括:CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD;免疫原为CatNo:Ag0226Product name: Recombinant human CFB proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 574-762 aa ofBC007990Sequence: DYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLG种属同源性预测;储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.,储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.;产品形式为Liquid;纯化方式为Antigen affinity purification。

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文献和实验Polyclonal Antibody Production
to inactivate complement. Ring the inside walls of the tube with a glass pipet and place at 4 °C overnight to clot. Decant the clotted blood into a centrifuge tube and centrifuge at 4 °C 10,000g for 10
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b
and place it in its cage. Take the collected blood and place at 37°C for 30 minutes to inactivate complement. Place the tube at 4°C overnight to clot. Loosen the clot from the tube wall with a spatula and decant the blood into a plastic centrifuge tube
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