CBX5 Polyclonal antibody

【产品概述】
本产品为CBX5 Polyclonal antibody（货号：11831-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse, rat来源的靶蛋白，经测试适用于WB, IP, IHC, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。

【背景介绍】
Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443). This antibody is a rabbit polyclonal antibody raised against a full-length human HP1A protein ,and can reacts with the 28kd HP1A protein.

【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为25-30 kDa。推荐稀释比：WB: WB : 1:500-1:1000；IHC: IHC : 1:50-1:500；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为HeLa cells, human kidney tissue, K-562 cells, A431 cells, MCF-7 cells, HEK-293 cells。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。

【产品信息】
GenBank蛋白编号：BC006821；基因名称：CBX5；Gene ID (NCBI)：23468；UNIPROT ID：P45973；RRID：AB_2071356；别名包括：Antigen p25, CBX5, Chromobox protein homolog 5, HP1, HP1 alpha, HP1A；免疫原为CatNo:Ag2403Product name: Recombinant human CBX5 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 1-191 aa ofBC006821Sequence: MGKKTKRTADSSSSEDEEEYVVEKVLDRRVVKGQVEYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKYKKMKEGENNKPREKSESNKRKSNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。