Frizzled 7 Polyclonal antibody

【产品概述】
本产品为Frizzled 7 Polyclonal antibody（货号：16974-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse来源的靶蛋白，经测试适用于WB, IP, IHC, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。

【背景介绍】
Frizzled class receptor 7 (Frizzled 7, also known as FZD7) is a member of the superfamily of G protein-coupled receptor proteins that act as receptors in the Wnt signaling pathway. Frizzled 7 plays a role in development, stem cell renewal, and differentiation and has been implicated in the progression of various cancers. What is the molecular weight of Frizzled 7? Is Frizzled 7 post-translationally modified? The molecular size of Frizzled 7 is ~63 kDa, which represents the mature N-glycosylated species (PMID: 25274627). Frizzled 7 can form homodimers and heterodimers with other Frizzled family members (PMID: 14688793). What is the subcellular localization of Frizzled 7? Frizzled 7 is a seven-pass transmembrane protein that is present at the plasma membrane. In endothelial cells, it predominantly locates to cell-cell junctions (PMID: 24866384). Frizzled 7 has a cleavage site for calpain-1 protease in its C-terminus that regulates its activity and plasma membrane turnover (PMID: 17716656). What is the tissue expression pattern of Frizzled 7? Frizzled 7 is expressed at high levels in adult skeletal muscles and in fetal kidneys, but is also present in fetal lung, adult heart, brain, and placenta (PMID: 9813155). Frizzled 7 expression is increased in various cancer types, including melanoma, hepatocellular carcinoma, lung, esophageal, gastric, and colon cancers.

【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为64 kDa。推荐稀释比：WB: WB : 1:500-1:2000；IHC: IHC : 1:400-1:1600；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为mouse skeletal muscle tissue, mouse heart tissue, mouse kidney tissue。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。

【产品信息】
GenBank蛋白编号：BC015915；基因名称：Frizzled 7；Gene ID (NCBI)：8324；UNIPROT ID：O75084；RRID：AB_2294535；别名包括：Frizzled 7, Fz 7, FZD7, FzE3, hFz7；免疫原为CatNo:Ag9446Product name: Recombinant human FZD7 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 33-257 aa ofBC015915Sequence: QPYHGEKGISVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTAPYLPDLPFTALPPGASDGRGRPAFPFSCPRQLKVPPYLGYRFLGERDCGAPCEPGRANGLMYFKEEERRFARLWV种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。