- ￥300 - 3000
- proteintech
- 国内
- 10233-1-AP
- 2026年05月11日
- WB, IHC, IF/ICC, IF-P, IP, ELISA
- Rabbit / IgG
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
详询
- 亚型：
IgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
详询
- 供应商：
上海艾科斯生物科技有限公司
- 宿主：
Rabbit / IgG
- 应用范围：
WB, IHC, IF/ICC, IF-P, IP, ELISA
- 浓度：
400 ug/ml
- 靶点：
NDE1
- 抗体英文名：
NDE1
- 抗体名：
NDE1
- 规格：
50 μL/100 μL/150 μL
【产品概述】
本产品为NDE1 Polyclonal antibody（货号：10233-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse, rat来源的靶蛋白，经测试适用于WB, IHC, IF/ICC, IF-P, IP, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。
【背景介绍】
NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.
【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为40 kDa。推荐稀释比：WB: WB : 1:500-1:3000；IHC: IHC : 1:20-1:200；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为A2780 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse ovary tissue, mouse brain tissue, rat brain tissue。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。
【产品信息】
GenBank蛋白编号：BC001421；基因名称：NDE1；Gene ID (NCBI)：54820；UNIPROT ID：Q9NXR1；RRID：AB_2149877；别名包括：HOM TES 87, NudE, NUDE1；免疫原为CatNo:Ag0336Product name: Recombinant human NDE1 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 41-282 aa ofBC001421Sequence: EGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQS种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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