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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
IL-21, Human
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
10μg/50μg/1mg
| 规格: | 10μg | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥2200.0 |
| 规格: | 1mg | 产品价格: | ¥16800.0 |
| Species | Human | |
| Protein Construction |
Expressed with an N-terminal Met.
|
|
| Purity | > 95% as analyzed by SDS-PAGE | |
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | |
| Biological Activity | ED50≤ 3.0 ng/ml, measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. | |
| Expression System | E. coli | |
| Apparent Molecular Weight | ~15.4 kDa, on SDS-PAGE under reducing conditions. | |
| Formulation | Lyophilized after extensive dialysis against PBS. | |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. | |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background | Interleukin-21 (IL-21) belongs to the Type I four helix bundle cytokines, and shares the common cytokine receptor γ chain with IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is expressed by CD4+ T cells, natural killer (NK) T cells, and Th17 cells, and the IL-21 receptor is highly expressed on CD4+ and CD8+ B cells; indeed, IL-21 plays a pivotal role in the survival and proliferation of B cells, and their differentiation to immunoglobulin (Ig) producing cells. IL-21 up-regulates and down-regulates the production of IgG1 and IgE by B cells, respectively, and diminishes the severity of allergy and asthma. In some case, IL-21 also induces the apoptosis of B cells. The other roles of IL-21 include regulation of innate immune systems, implication on autoimmunity, and antitumor actions. |
| Synonyms | IL21; CVID11; IL-21; Za11; interleukin 21 |
产品特点:
1. 【人源天然序列】采用人源 HRG1-1 天然氨基酸序列,无突变,与体内天然蛋白结构完全一致,可特异性结合 HRG1-1 配体2. 【高活性高特异性】可特异性结合 HRG1-1 抗体,ED₅₀ < 100 ng/mL(抗体结合实验),可被 HRG1-1 特异性抗体识别,适用于抗体筛选、抗原检测3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配细胞培养、体内动物实验等敏感场景需求4. 【易纯化检测】哺乳动物细胞表达,可通过亲和层析快速纯化,也可通过 HRG1-1 特异性抗体进行检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 HRG1-1 蛋白结构与功能研究、细胞培养添加剂、抗体制备与筛选、药物靶点验证等
HRG1-1, 人源,人,重组蛋白,重组,高活性,高纯度,低内毒素,细胞培养,科研实验,哺乳动物细胞表达,Human,HRG1-1,Recombinant protein,High activity,High purity,Low endotoxin,Cell culture,Mammalian expression
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文献和实验Human IL-2 Enzyme Immunoassay (EIA)
Increased division and maturation of activated helper T cells Stimulation of neutrophils Stimulus for the secretion of interferon-( and tumor necrosis factors - aphla and - Beta from peripheral mononuclear cells. The Endogen Human IL-
Evaluation of IL-2-Withdrawal-Induced Apoptosis in Human T Lymphocytes
pathway of lymphocyte apoptosis triggered by starvation for IL-2 or other survival cytokines.
我们知道,PeproTech的所有细胞因子和蛋白均为冻干粉,这使得运输非常便捷,只要常温即可。而且,细胞因子和蛋白冻干粉非常稳定,在-20℃或-80℃条件下可保存数年。冻干粉在使用前需进行溶解,然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键,因溶解不好会导致细胞因子或蛋白的失活,这也是很多用户在实际使用中经常遇到的问题。 那么,应该如何进行正确的溶解呢? 下面我们以Recombinant Human IL-4 (重组人IL-4,产品编号:200-04)的说明书为例
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