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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
FGF-acidic, Mouse
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
10μg/50μg/1mg
| 规格: | 10μg | 产品价格: | ¥300.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥600.0 |
| 规格: | 1mg | 产品价格: | ¥5000.0 |
| Species | Mouse | |
| Protein Construction |
|
|
| Purity | > 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC |
|
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | |
| Biological Activity | ED50 < 0.4 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 10.0 µg/ml heparin, corresponding to a specific activity of > 2.5 × 106 U/mg. | |
| Expression System | E. coli | |
| Apparent Molecular Weight | ~15.8 kDa, on SDS-PAGE under reducing conditions. | |
| Formulation | Lyophilized after extensive dialysis against PBS. | |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. | |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background | Fibroblast Growth Factor- acidic (FGF-acidic) is a mitogen targeting at the endothelial cells, and belongs to the heparin binding FGF family, which contains 22 members. FGF-acidic binds to the receptor family FGFR1-4 in vivo with the assistance of heparin. However, along with FGF -basic, FGF-acidic lacks the signal peptide segment, and thus is not secreted via endoplasmic reticulum (ER) and Golgi bodies. Studies have shown that FGF-acidic is highly regulated, and it is a direct angiogenesis factor. If unregulated, angiogenesis could contribute to several diseases including arthritis, diabetes, ocular neovascularization, and especially tumors. Therefore, FGF-acidic is treated as a potential oncogene, and its overexpression is correlated tightly with several cancers. |
| Synonyms | Fibroblast Growth Factor-acidic; HBGF-1; ECGF-beta; FIBP; FGFIBP; FIBP-1; ECGF; ECGFA; GLIO703; FGF1; FGF-a |
产品特点:
1. 【人源天然序列】采用人源 NGF-R 天然氨基酸序列,无突变,与体内天然蛋白结构完全一致,可特异性结合 NGF 配体2. 【高活性高特异性】可高效结合 NGF 配体,ED₅₀ < 100 ng/mL(配体结合实验),可被 NGF-R 特异性抗体识别,适用于抗体筛选、神经发育机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配细胞培养、体内神经发育实验等敏感场景需求4. 【易纯化检测】哺乳动物细胞表达,可通过亲和层析快速纯化,也可通过 NGF-R 特异性抗体进行检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 NGF-R 蛋白结构与功能研究、神经发育与再生机制研究、细胞培养添加剂、药物筛选、抗体研发等
NGF-R, 神经生长因子受体,人源,人,重组蛋白,重组,高活性,高纯度,低内毒素,细胞培养,科研实验,哺乳动物细胞表达,Human,NGF-R,Recombinant protein,Nerve growth factor receptor,High activity,High purity,Low endotoxin,Cell culture,Mammalian expression
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