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- ￥5800 - 19200
- 上海经科
- 中国
- JK7583
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
3 months at -20°C
- 英文名：
PD-L1/B7-H1[Biotin], His & Avi, Human
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
100μg/500μg
| 规格： | 100μg | 产品价格： | ￥5800.0 |
|---|---|---|---|
| 规格： | 500μg | 产品价格： | ￥19200.0 |
| Species | Human | ||||||
| Protein Construction |
|
||||||
| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 28.1 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
| Synonyms | CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
产品特点：
1. 【人源天然序列】采用人源 PD-L1（B7-H1）天然氨基酸序列，无突变，与体内天然蛋白结构完全一致，可特异性结合 PD-1 配体2. 【高活性高特异性】可高效结合 PD-1 配体，ED₅₀ < 100 ng/mL（配体结合实验），可被 PD-L1 特异性抗体识别，适用于抗体筛选、免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%，内毒素 < 0.2 EU/μg（LAL 法），适配免疫细胞培养、体内免疫实验等敏感场景需求4. 【多标签易纯化检测】哺乳动物细胞表达，N 端带 Avi 标签、C 端带 His 标签，可通过 Ni-NTA 亲和层析快速纯化，也可通过 Avi/His 标签抗体、生物素亲和素系统进行特异性检测5. 【稳定易保存】蛋白储存液配方优化，未开封 - 80℃可稳定保存 12 个月，-20℃可存 6 个月，避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 PD-L1 蛋白结构与功能研究、免疫调控与免疫耐受机制研究、肿瘤免疫治疗研究、细胞培养添加剂、药物筛选、抗体研发等
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