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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
IL-21[Biotin], His & Avi, Human
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥5800.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥19200.0 |
| Species | Human | ||||||
| Protein Construction |
|
||||||
| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | E.coli | ||||||
| Theoretical Molecular Weight | 18.30 kDa | ||||||
| Apparent Molecular Weight | The protein has a predicted MW of 18.30 kDa same as Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in 20mM NaAc, 500mM NaCl (pH 5.5). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 500mM NaCl (pH 5.5). | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases. |
| Synonyms | Interleukin-21; IL-21; Za11; CVID11 |
产品特点:
1. 【人源天然序列】采用人源 IL-21 天然氨基酸序列,无突变,与体内天然蛋白结构完全一致,可特异性结合 IL-21 受体2. 【高活性高特异性】可高效结合 IL-21 受体,ED₅₀ < 100 ng/mL(受体结合实验),可被 IL-21 特异性抗体识别,适用于抗体筛选、免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内免疫实验等敏感场景需求4. 【多标签易纯化检测】哺乳动物细胞表达,N 端带 Avi 标签、C 端带 His 标签,可通过 Ni-NTA 亲和层析快速纯化,也可通过 Avi/His 标签抗体、生物素亲和素系统进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 IL-21 蛋白结构与功能研究、免疫调控与炎症机制研究、肿瘤免疫治疗研究、细胞培养添加剂、药物筛选、抗体研发等
IL-21, 白细胞介素 21, 人源,人,重组蛋白,重组,高活性,高纯度,低内毒素,生物素标记,His 标签,Avi 标签,细胞培养,免疫细胞,科研实验,哺乳动物细胞表达,Human,IL-21,Recombinant protein,IL-21,Biotin conjugated,His-tagged,Avi-tagged,High activity,High purity,Low endotoxin,Cell culture,Immune cell,Mammalian expression
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文献和实验QIAgene Expression Kit——再也不为重组蛋白的表达效率发愁 据Protein Database的最新数据表明,目前已知的人类蛋白有35,000种(包括splice variants),大约33%的蛋白正处于研究中,但是只有8%的蛋白能够成功表达。统计数据同时显示,目前克隆,表达,纯化的成功率只维持在54%,74%,59%。所以说,在蛋白表达的漫漫征途中,拿不到想要的蛋白是一件非常普遍的事情。举一个例子来说对于目标蛋白不表达或表达量低这个常见问题,需要排除或检查的因素就太多
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