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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
BTN3A1/CD277[Biotin], His & Avi, Cynomolgus
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥5800.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥19200.0 |
| Species | Cynomolgus | ||||||
| Protein Construction |
|
||||||
| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 26.5 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg). |
| Synonyms | CD277; BTN3A1; BTF5; BT3.1; BTN3.1 |
产品特点:
1. 【食蟹猴源天然序列】采用食蟹猴源 BTN3A1/CD277 天然氨基酸序列,无突变,与天然蛋白结构完全一致,可特异性结合 BTN3A1 配体2. 【高活性高特异性】可特异性结合 BTN3A1 抗体,ED₅₀ < 100 ng/mL(抗体结合实验),可被 BTN3A1 特异性抗体识别,适用于抗体筛选、免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内动物实验等敏感场景需求4. 【三标签易纯化检测】N 端带 Avi 标签、C 端带 His 标签,同时可实现生物素标记,可通过 Ni-NTA 亲和层析快速纯化,也可通过标签抗体、生物素亲和素系统进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 BTN3A1 蛋白结构与功能研究、免疫调控机制研究、抗体制备与筛选、药物靶点验证、细胞培养添加剂等
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