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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
B7-1/CD80, His, Cynomolgus
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥2600.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥8800.0 |
| Species | Cynomolgus | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 25 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. |
| Synonyms | CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7 |
产品特点:
1. 【食蟹猴源天然序列】采用食蟹猴源 B7-1/CD80 天然氨基酸序列,无突变,与天然蛋白结构完全一致,可特异性结合 CD28/CTLA-4 配体2. 【高活性高特异性】可特异性结合 CD80 抗体,ED₅₀ < 100 ng/mL(抗体结合实验),可被 CD80 特异性抗体识别,适用于抗体筛选、免疫检查点机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内动物实验等敏感场景需求4. 【His 标签易纯化检测】C 端带 His 标签,可通过 Ni-NTA 亲和层析快速纯化,也可通过 His 标签抗体进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 CD80 蛋白结构与功能研究、免疫检查点与 T 细胞活化机制研究、抗体制备与筛选、药物靶点验证、细胞培养添加剂等
B7-1,CD80, 食蟹猴源,食蟹猴,重组蛋白,重组,高活性,高纯度,低内毒素,His 标签,免疫检查点,细胞培养,科研实验,哺乳动物细胞表达 Cynomolgus,B7-1,CD80,Recombinant protein,His-tagged,High activity,High purity,Low endotoxin,Immune checkpoint,Cell culture,Mammalian expression
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文献和实验共刺激作用。八十年代末至九十年代初期共刺激分子B7在肿瘤免疫研究中较为深入和广泛。 共刺激分子B7为44-54kD的糖蛋白,属于免疫球蛋白超家族成员。最初发现于活化的B细胞上,它也表达于树突状细胞、活化的巨噬细胞上。除B细胞来源的肿瘤外,其他肿瘤很少表达B7。共刺激分子的缺乏也是肿瘤的弱免疫原性的重要因素。B7又分为B7-1(又名为CD80)和B7-2(又名为CD86),两者仅有25%氨基酸同源。APC活化时,B7-2表达早于 B7-1。B7有两种受体存在于T细胞表面,CD28低亲和力受体
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