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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
HLA-A*01:01&B2M&CT83 (NTDNNLAVY) Monomer [Biotin], His&Avi, Human
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥4500.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥14800.0 |
| Species | Human | ||||||
| Protein Construction |
|
||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 50.30 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background | Cancer/testis antigens 83 (CT83), also called KK-LC-1 or CXorf61, recognized by cytotoxic T lymphocytes (CTL), has become a promising target for immunotherapy. |
| Synonyms | CT83; HLA class I; MHC class I; HLA-A; HLA-A*0101; MHC; MHC HLA-A alpha; NTDNNLAVY |
产品特点:
1. 【人源天然序列】采用人源 HLA-A*01:01、B2M 天然氨基酸序列,结合 CT83 NTDNNLAVY 抗原肽,与体内天然 HLA 单体结构完全一致,可特异性结合 T 细胞受体2. 【高活性高特异性】可特异性结合对应 T 细胞受体,ED₅₀ < 100 ng/mL(T 细胞结合实验),可被 HLA 特异性抗体识别,适用于 T 细胞筛选、抗原特异性免疫研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内免疫实验等敏感场景需求4. 【三标签易纯化检测】N 端带 Avi 标签、C 端带 His 标签,同时可实现生物素标记,可通过 Ni-NTA 亲和层析快速纯化,也可通过标签抗体、生物素亲和素系统进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 HLA 蛋白结构与功能研究、CT83 抗原特异性 T 细胞免疫机制研究、肿瘤免疫治疗研究、细胞培养添加剂、药物筛选、抗体研发等
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