- ￥252 - 4284
- AmBeed
- A210855
- 2026年05月28日
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- 询价记录
- 文献和实验
- 技术资料
- 英文名：
7-Oxo-7H-benzo[de]benzo[4,5]imidazo[2,1-a]isoquinoline-3-carboxylic acid
- 供应商：
上海安毕达生物科技有限公司
- CAS号：
52029-86-4
- 规格：
1mg/5mg/10mg/25mg/50mg/100mg
| 规格： | 1mg | 产品价格： | ￥252.0 |
|---|---|---|---|
| 规格： | 5mg | 产品价格： | ￥504.0 |
| 规格： | 10mg | 产品价格： | ￥806.0 |
| 规格： | 25mg | 产品价格： | ￥1694.0 |
| 规格： | 50mg | 产品价格： | ￥2709.0 |
| 规格： | 100mg | 产品价格： | ￥4284.0 |
STO-609 suppresses the functionality of both recombinant CaM-KKα and CaM-KKβ isoforms, exhibiting Ki measurements of 80 and 15 ng/mL for each, respectively, and hampers their self-phosphorylation processes. It demonstrates a pronounced selectivity towards CaM-KK, showing negligible influence on the subsequent CaM kinases (CaM-KI and -IV), with the IC50 against CaM-KII being 10 μg/mL. Both the constitutively active and the wild-type variants of CaM-KKα are inhibited by STO-609. It also curtails the Ca2+-prompted activation of CaM-KIV within HeLa cells that have undergone transfection, operating in a concentration-dependent fashion. Moreover, at a dose of 1μg/mL, STO-609 markedly lowers the inherent activity of CaM-KK in SH-SY5Y neuroblastoma cells, achieving an inhibition rate of 80% 1.
STO-609 suppresses the functionality of both recombinant CaM-KKα and CaM-KKβ isoforms, exhibiting Ki measurements of 80 and 15 ng/mL for each, respectively, and hampers their self-phosphorylation processes. It demonstrates a pronounced selectivity towards CaM-KK, showing negligible influence on the subsequent CaM kinases (CaM-KI and -IV), with the IC50 against CaM-KII being 10 μg/mL. Both the constitutively active and the wild-type variants of CaM-KKα are inhibited by STO-609. It also curtails the Ca2+-prompted activation of CaM-KIV within HeLa cells that have undergone transfection, operating in a concentration-dependent fashion. Moreover, at a dose of 1μg/mL, STO-609 markedly lowers the inherent activity of CaM-KK in SH-SY5Y neuroblastoma cells, achieving an inhibition rate of 80% 1.
溶解方案(细胞实验)
DMSO 中的溶解度 : 30 mg/mL (95.45 mM; 超声助溶 (<80°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)
溶解方案(动物实验)
"方案一": "请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% SalineSolubility: ≥ 0.1 mg/mL (0.32 mM); 澄清溶液 此方案可获得 ≥ 0.1 mg/mL（饱和度未知）的澄清溶液。以 1 mL 工作液为例，取 100 μL 1.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。"
"方案二": "请依序添加每种溶剂：10% DMSO 90% (20% SBE-β-CD in Saline)Solubility: ≥ 0.1 mg/mL (0.32 mM); 澄清溶液 此方案可获得 ≥ 0.1 mg/mL（饱和度未知）的澄清溶液。以 1 mL 工作液为例，取 100 μL 1.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。"
"方案一": "请依序添加每种溶剂：50% PEG300 50% SalineSolubility: 0.5 mg/mL (1.59 mM); 悬浊液; 超声助溶"
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文献和实验。 我对此专门做过测试，RT下放1小时，基本无影响，4小时后开始有降解。 4hr－RT表示在RT下放 4hr-RT-sto表示在RT下放在我研制的RNA保护剂中放置4小时 husttjmu zjubell，非常感谢你的帮助！我心里有底啦，谢谢！ 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你，都可以成为师兄的好伙伴 师兄微信号：shixiongcoming
ES Cell Culture and Manipulation
them when a colony is approximately 1-2 times the diameter of a stretched out fibroblast (STO cell). If the plate is densely covered with colonies, they may be split 1:10, or slightly greater. If there are a lot of colonies, split them 1:6-1:8. For a plate
ES Cell Culture and Manipulation
(STO cell). If the plate is densely covered with colonies, they may be split 1:10, or slightly greater. If there are a lot of colonies, split them 1:6-1:8. For a plate that has only a few colonies (12-20), allow them to get slightly larger than normal
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