4E2RCat

4E2RCat obstructs the binding between eIF4E, the cap-binding element, and eIF4G, the extensive scaffolding molecule, thereby halting cap-dependent translation. This compound notably curtails the replication of HCoV-229E, leading to a reduction in both the proportion of cells infected and the titers of infectious virus inside and outside the cells. The inhibition of cap-dependent translation by 4E2RCat is contingent on the dosage. While it impedes cap-dependent FF translation, it does not affect EMCV IRES-mediated Ren translation. The suppression of coronavirus replication by 4E2RCat is influenced by both the concentration and duration of exposure¹.

溶解方案(细胞实验)

DMSO 中的溶解度 : 23.33 mg/mL (51.17 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

溶解方案(动物实验)

"方案 一": "请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% SalineSolubility: 2.33 mg/mL (5.11 mM); 悬浊液; 超声助溶 此方案可获得 2.33 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。以 1 mL 工作液为例，取 100 μL 23.3 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。"