- ￥1176 - 9828
- abinScience
- France
- HA195033
- 2026年04月27日
- WB: 1:500-1:2000
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 形态：
Liquid
- 保存条件：
-20°C
- 克隆性：
单克隆
- 保质期：
twelve months
- 级别：
一抗
- 供应商：
武汉研升生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB: 1:500-1:2000
- 抗体英文名：
Anti-Gamma-protocadherin-B2 Antibody (N148/5)
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1176.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1956.0 |
| 规格： | 1mg | 产品价格： | ￥9828.0 |
| 产品名 | Anti-Gamma-protocadherin-B2 Antibody (N148/5) |
| 货号 | HA195033 |
| 宿主 | Mouse |
| 克隆类型 | Monoclonal |
| 克隆号 | N148/5 |
| 靶标 | PCDHGB2, PCDH-gamma-B2, Protocadherin gamma-B2 |
| 同种型 | IgG2a, kappa |
| 应用 | IHC, WB |
| 应用范围 | WB: 1:500-1:2000 |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |
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文献和实验with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C. • To purify the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes. • Wash with 50 ml 10 mM Tris
Antibody Humanization by CDR Grafting
of antibody therapeutics in the 1980s, when the major obstacle was the human anti-murine antibody (HAMA) response (1 , 2 ). The HAMA response occurred in up to 50% of patients upon the administration of murine hybridoma-derived antibodies (3 ) and severely
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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