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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.063 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠活化T-细胞核因子1(NFATC1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat NFATC1(Nuclear Factor Of Activated T-Cells, Cytoplasmic 1) ELISA Kit |
| 别名 | NF-ATC; NFAT2; NFATc; NFAT transcription complex cytosolic component |
| 货号 | ELK9692 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 0.063 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NFATC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NFATC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NFATC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NFATC1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Infection immunity; |

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文献和实验Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
Activation of Nuclear Factor-B
Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB
Nuclear Recruitment Assay as a Tool to Validate Transcription Factor Interactions in Mammalian Cells
Identification and verification of novel transcription factor interactions is an inherent step in the discovery of molecular mechanisms driving gene transcription and regulation. Co-immunoprecipitation and GST-pull down are often key
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