大鼠肌球蛋白重链8(MYH8)微量上样酶联免疫吸附检测试剂盒
【Rat MYH8(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal) Microsample ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 62.5-4000 pg/mL
- 24.7 pg/mL
- 武汉
- ELK6559MS
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
62.5-4000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
Serum, plasma and other biological fluids.
- 灵敏度：
24.7 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 大鼠肌球蛋白重链8(MYH8)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat MYH8(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal) Microsample ELISA Kit |
| 别名 | MyHC-peri; MyHC-pn; MyHC-perinatal; Myosin heavy chain, skeletal muscle, perinatal |
| 货号 | ELK6559MS |
| 反应种属 | Rat |
| P04462 | P04462 |
| 检测类型 | Sandwich |
| 灵敏度 | 24.7 pg/mL |
| 标准品 | 4000 pg/mL |
| 检测范围 | 62.5-4000 pg/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYH8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYH8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYH8 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway; |
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文献和实验Protein purification: skeletal muscle myosin
Overview SKELETAL MUSCLE MYOSIN A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion
Myosin Light Chain Preparation
.Urea Homogenizing Buffer: 2.4 liters: 8 M urea,1 mM EDTA,15 mM MCE,20 mM Tris (2.4 liters 8 M urea,12 ml 200 mM EDTA,2.4 ml 15 M MCE,24 ml 2 M Tris) Buffer D.ETOH/MCE: 3 liters: For skeletal Muscle- 95% ETOH,15 mM MCE (3 liters ETOH,3 ml 15 M MCE
Purification of Myosin Light Chain Kinase
should be injected (ear vein)with ~5 cc of nembutal solution and then exsanguinated and skinned.Skeletal muscle should be cleaned of fat and connective tissue and kept on ice. 2.The tissue should be ground in the cold room using the fine setting of the meat grinder
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