- ￥1960 - 2800
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.058 ng/mL
- 武汉
- ELK6519
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.058 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 大鼠线粒体解偶联蛋白2(UCP2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat UCP2(Uncoupling Protein 2, Mitochondrial) ELISA Kit |
| 别名 | SLC25A8; UCPH; Solute carrier family 25 member 8 |
| 货号 | ELK6519 |
| 反应种属 | Rat |
| P56500 | P56500 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.058 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat UCP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat UCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UCP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Developmental science; |
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文献和实验Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in �regulating apoptosis in different cell systems
, mitochondrial uncoupling proteins (UCPs) displayed (non-native) artefactual uncoupling when expressed in yeast or can possess functions upon reconstitution in proteoliposomes that cannot be reproduced in isolated mitochondria. In the light of newly discovered
酶免疫测定（enzyme immunoassay, EIA）或免疫酶技术（immunoenzymatic technique）是指用酶标记抗体或酶标记抗体进行的抗原抗体反应。它采用抗原与抗体的特异反应与酶连接，然后通过酶与底物产生颜色反应，用于定量测定。目前常用的方法称为酶联免疫吸附法（enzyme-linked immunosorbent assay, ELISA），其方法简单，方便讯速，特异性强。ELISA是由抗原（抗体）先结合在固相
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