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小鼠DNA甲基转移酶3B(DNMT3B)酶联免疫吸附检测试剂

盒【Mouse DNMT3B(DNA Methyltransferase 3B) ELISA Kit】
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  • ¥1960 - 2800
  • ELK Biotechnology
  • 0.16-10 ng/mL
  • 0.045 ng/mL
  • 武汉
  • ELK6118
  • 2026年05月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      武汉研升生物科有限公司

    • 检测范围

      0.16-10 ng/mL

    • 检测方法

      Sandwich

    • 适应物种

      Mouse

    • 样本

      Tissue homogenates and other biological fluids.

    • 灵敏度

      0.045 ng/mL

    • 规格

      48T/96T

    规格:48T产品价格:¥1960.0
    规格:96T产品价格:¥2800.0
    中文名称 小鼠DNA甲基转移酶3B(DNMT3B)酶联免疫吸附检测试剂盒
    英文名称 Mouse DNMT3B(DNA Methyltransferase 3B) ELISA Kit
    别名 ICF; M.HsaIIIB; DNA methyltransferase HsaIIIB
    货号 ELK6118
    反应种属 Mouse
    O88509 O88509
    检测类型 Sandwich
    灵敏度 0.045 ng/mL
    标准品 10 ng/mL
    检测范围 0.16-10 ng/mL
    样本类型 Tissue homogenates and other biological fluids.
    反应时间 3.5h
    检测原理 The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DNMT3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DNMT3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DNMT3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DNMT3B in the samples is then determined by comparing the OD of the samples to the standard curve.
    研究领域 Enzyme & Kinase;

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    图标文献和实验
    相关实验
    • DNA甲基化的介绍及其抑制剂

      ; 2)DNM T3a、DNM T3b从头甲基转移酶, 它们可甲基化CPG, 使其半甲基化, 继而全甲基化。从头甲基转移酶可能参与细胞生长分化调控, 其中DNM T3b在肿瘤基因甲基化中起重要作用。三、 相关图解四、 DNMT的抑制剂Decitabine:Decitabine是一种DNA甲基化的有效抑制剂,作用于HL-60和KG1a细胞时,IC50分别为100 ng/mL和1 ng/mL。Lomeguatrib:Lomeguatrib是一种有效的O6-alkylguanine-DNA

    • Relative Quantitation of DNA Methyltransferase mRNA by Real-Time RT-PCR Assay

      by at least four DNA methyltransferase (Dnmt) enzymes, including Dnmt1, Dnmt2, Dnmt3a, and Dnmt3b. To understand fully how DNA methylation is involved in gene regulation, knowledge of Dnmt mRNA transcript levels is required, both as a surrogate measure of Dnmt protein

    • Inhibition of DNA Methylation in Somatic Cells

      the methylation marks through cell division. However, the de novo methyltransferases, Dnmt3a and Dnmt3b, can also contribute to the maintenance of the methylation pattern. Manipulation of these enzymes, especially Dnmt1, provides a means to alter DNA methylation

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    小鼠DNA甲基转移酶3B(DNMT3B)酶联免疫吸附检测试剂盒【Mouse DNMT3B(DNA Methyltransferase 3B) ELISA Kit】
    ¥1960 - 2800