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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Pig
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.122 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 猪甲胎蛋白(aFP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Pig aFP(Alpha-Fetoprotein) ELISA Kit |
| 别名 | A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein |
| 货号 | ELK5885 |
| 反应种属 | Pig |
| Q8MJ76 | Q8MJ76 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.122 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig aFP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig aFP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig aFP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig aFP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity;Hepatology; |

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文献和实验癌、卵巢肿瘤、恶性畸胎瘤、胰腺癌、肠癌、肺癌等患者。 产甲胎蛋白的胃癌 (alpha-fetoprotein-producing gastric carcinoma, AFPGC) 是一种相对少见的胃癌 (GC),最早由 Bourreille 等人 [6] 于 1970 年提出,高 AFP 水平的胃癌被称为 AFPGC [7]。AFPGC 约占 GC 的 2.7~8%[8],也有报道占 GC 的 5.1%~15%[9]。AFPGC 是指经过病理或组织学检查确诊,伴有血清 AFP 升高,并可以排除肝炎
甲胎蛋白(α-fetoprotein,αFP或AFP)主要在胎儿肝中合成,分子量6.9万,在胎儿13周AFP占血浆蛋白总量的1/3。在妊娠30周达最高峰,以后逐渐下降,出生时血浆中浓度为高峰期的1%左右,约40mg/L,在周岁时接近成人水平(低于30μmg/L)。 甲胎蛋白在产妇羊水或母体血浆中AFP可用于胎儿产前监测。如在神经管缺损、脊柱裂、无脑儿等时,AFP可由开放的神经管进入羊水而导致其在羊水中含量显著升高。胎儿在宫腔内死亡、畸胎瘤等先天缺陷亦可有羊水中AFP
北京大学和海南医学院联合对肝癌细胞特异性标志物甲胎蛋白(AFP)进行长时间的研究,发现AFP具有抑制PTEN的生物学功能,导致肝癌细胞耐受全反式维甲酸诱导的凋亡,这是AFP新功能的发现。近日以题为“Alpha-fetoprotein: a new member of intracellular signal molecules in regulation of the PI3K/AKT signaling in human hepatoma
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