牛组织金属蛋白酶抑制因子4(TIMP4)酶联免疫吸附检测试剂
盒【Cattle TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.127 ng/mL
- 武汉
- ELK5853
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Cattle
- 样本：
Serum, plasma and other biological fluids.
- 灵敏度：
0.127 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 牛组织金属蛋白酶抑制因子4(TIMP4)酶联免疫吸附检测试剂盒 |
| 英文名称 | Cattle TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit |
| 别名 | TIMP Metallopeptidase Inhibitor 4; Tissue Inhibitor of Metalloproteinases 4 |
| 货号 | ELK5853 |
| 反应种属 | Cattle |
| O97563 | O97563 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.127 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TIMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TIMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TIMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TIMP4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity;Cardiovascular biology; |
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文献和实验相关实验
多种细胞表达的基质金属蛋白酶。为探讨雌二醇（E2）对人成骨肉瘤MG-63细胞株和正常成人成骨细胞（hOB）膜型基质金属蛋白酶（MT1-MMP）的作用和绝经后骨质疏松症（OP）的作用机制。方法：Northern杂交、Western杂交和激光共聚焦显微系统免疫荧光法分别检测MT1-MMP mRNA和蛋白质表达。MMP-2活性用明胶酶谱和酶联免疫吸附测定检测。结果 首次观察到E2促进MG-63细胞MT1-MMPmRNA表达，并呈剂量依赖关系。E2促进MG-63细胞MT1-MMP蛋白质表达，并呈剂量
Microarray Database http://genome-www.stanford.edu/microarray Raw and normalized data from microarray experiments 34. Tooth Development Database http://bite-it.helsinki.fi/ Gene expression in dental tissue 35. TRANSPATH http://www.biobase.de/pages
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牛组织金属蛋白酶抑制因子4(TIMP4)酶联免疫吸附检测试剂盒【Cattle TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit】
￥1960 - 2800
