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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Guinea pig
- 样本:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 灵敏度:
0.054 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 豚鼠神经生长因子(NGF)酶联免疫吸附检测试剂盒 |
| 英文名称 | Guinea pig NGF(Nerve Growth Factor) ELISA Kit |
| 别名 | NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor |
| 货号 | ELK5818 |
| 反应种属 | Guinea pig |
| P19093 | P19093 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.054 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig NGF in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Neuro science; |

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文献和实验略称NGF。在将小鼠肉瘤180移植于3日龄鸡胚体壁时,与移植片连接的脊髓感觉神经节及交感神经节增大20%—40%,基于比克尔(E.D.Bueker,1948)的这一发现,科恩(S.Cohen,1954)等从小鼠肉瘤180和37中成功地分离出具有同一活性的核蛋白质,以后从蛇毒中分离出具有千倍活性(Cohen,R.Levi-Montalcini,1956)和从小鼠颚下腺分离出具有万倍活性的蛋白质(Cohen,1960),这种蛋白质被称为神经生长因子。科恩等认为,其有效成分是分子量约为2.2万
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠神经生长因子 ( NGF )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 NGF 单抗包被于酶标板上,标准品和样品中的 NGF与单抗结合,加入生物素化的抗豚鼠 NGF
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
CalbiochemReferenceWeskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journal of Neurochemistry, 48 (6),1779-1786.
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