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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Dog
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
5.9 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 犬白介素13(IL13)酶联免疫吸附检测试剂盒 |
| 英文名称 | Dog IL13(Interleukin 13) ELISA Kit |
| 别名 | IL-13;ALRH; BHR1; P600;NC30 |
| 货号 | ELK5800 |
| 反应种属 | Dog |
| Q9N0W9 | Q9N0W9 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.9 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IL13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IL13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IL13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IL13 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Infection immunity; |

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文献和实验【讨论】Innate immunity: 'Natural helper' cells identified
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AHPGWFICTS CNCNEPVGVT DKFENRKHIEFSFQPVCKAE MSPSEVSD2, FeaturesDL-IL1z-Hu人白介素 1ζ(IL1Z) 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL1Z 含量。标本的采集与保存1. 血清:将收集于血清分离管的全血标本在室温放置 2 小时或 4℃ 过夜,然后 1000×g 离心 20 分钟,取上清即可,或将上清置于-20℃ 或-80
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