大鼠心型脂肪酸结合蛋白(FABP3)微量上样酶联免疫吸附检测
试剂盒【Rat FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) Microsample ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.79-50 ng/mL
- 0.32 ng/mL
- 武汉
- ELK5759MS
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.79-50 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.32 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 大鼠心型脂肪酸结合蛋白(FABP3)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) Microsample ELISA Kit |
| 别名 | MDGI; FABP11; H-FABP; O-FABP; Hfabp; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-type fatty acid-binding protein |
| 货号 | ELK5759MS |
| 反应种属 | Rat |
| P07483 | P07483 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.32 ng/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.79-50 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FABP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FABP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FABP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FABP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway;Cardiovascular biology; |
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文献和实验the concentration of unbound FA is a method using the fluorescent-(acrylodan) labeled intestinal FA-binding protein (ADIFAB). Under appropriate conditions, ADIFAB fluorescence measures unbound FA, regardless of any third phase such as albumin, FA-binding proteins
Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
The authors’ laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays
A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening
that bind to human fatty acid amide hydrolase (FAAH). This SPA assay utilizes the specific binding of [3 H]-R (+)-methanandamide (3 H-MAEA), a competitive nonhydrolyzed FAAH inhibitor, to FAAH expressing microsomes and evaluates its displacement by FAAH
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