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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Dog
- 样本:
Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids.
- 灵敏度:
5.5 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 犬转化生长因子β1(TGFb1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Dog TGFb1(Transforming Growth Factor Beta 1) ELISA Kit |
| 别名 | TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide |
| 货号 | ELK5702 |
| 反应种属 | Dog |
| P54831 | P54831 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.5 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Tumor immunity;Infection immunity; |

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文献和实验的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
剂测定(enzyme linked immunosorbent assay ),简称ELISA ,在国内有译作酶联免疫吸附试验的,虽然含义不完全确切,但已习用。
我最近在网上看到有公司在ELISA试剂盒的促销,好划算啊 我想把这个好消息,好资源与大家分享 几年来,博士德的ELISA试剂盒以优良的品质、卓越的性价比和良好的售后服务受到广大科研工作者的青睐。更因其品种齐全、操作简单、检测时间短、所需样品少而成为细胞因子检测的经典产品。公司生产的近270多种 ELISA 试剂盒,涉及分子生物学、分子免疫学、细胞生物学等生命科学研究的各个领域。公司总部位于武汉,代理商经销商遍及全国各地,形成了覆盖全国的有效服务网络,训练有素的专业人员能为客户提供技术
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