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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
5.8 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠轴突生长诱向因子1(Ntn1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat Ntn1(Netrin 1) ELISA Kit |
| 别名 | NTN1L; Epididymis tissue protein Li 131P |
| 货号 | ELK5693 |
| 反应种属 | Rat |
| Q924Z9 | Q924Z9 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.8 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Ntn1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Ntn1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Ntn1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Ntn1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |

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,平均批间变异系数为11.4%;液相芯片与ELISA法检测结果的相关系数R2为0.88~0.99。 国外多家公司已推出了商品化的细胞因子液相芯片检测试剂盒,比如R&D 公司的Flurokine MAP系列、Linco Research公司的LINCOplex 试剂盒、BioSource公司的Antibody Bead Kit、Bio Rad公司的Bio Plex系列等,可用于来自人、小鼠、大鼠的样品,既有一次检测单种细胞因子的试剂盒,也有预先混合多种细胞因子抗体交联微球而一次检测多个指标
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