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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.058 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人Diablo同源物(DIABLO)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human DIABLO(Diablo Homolog) ELISA Kit |
| 别名 | SMAC; DIABLO-S; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase |
| 货号 | ELK4816 |
| 反应种属 | Human |
| Q9NR28 | Q9NR28 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.058 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DIABLO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DIABLO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DIABLO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DIABLO in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验(Bax, Bak, Bok/Mtd, Bcl-rambo) into heterodimeric units or 'pores' in the outer mitochondrial membrane. This causes the release of numerous 'apoptogenic' factors, which include cytochrome c, Smac/DIABLO, apoptosis-inducing factor (Aif
Fluorometric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis
The mitochondrial regulation of cell death involves the release of proapoptotic factors, such as cytochrome c , Smac-DIABLO, AIF, OMI/HtrA2, by disruption of the outer mitochondrial membrane (OMM) permeability barrier that is controlled
跨膜电位,线粒体膜间隙的促凋亡蛋白质如细胞色素c、Smac/ Diablo 、Omi/Ht rA2 、凋亡诱导因子(AIF) 和核酸内切酶G.等 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴 师兄微信号:shixiongcoming
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