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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
1.57-100 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.67 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人谷胱甘肽过氧化酶4(GPX4)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human GPX4(Glutathione Peroxidase 4) ELISA Kit |
| 别名 | MCSP; PHGPx; SnGPx; SnPHGPx; Phospholipid Hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial |
| 货号 | ELK4775 |
| 反应种属 | Human |
| P36969 | P36969 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.67 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPX4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPX4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPX4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPX4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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文献和实验的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
Oxford Biomedical Research的氧化应激及慢性炎症生物标志物检测
., Roberts II, L.J., Meth. Enz. 300: 3-12 3. 肌酸酐检测试剂盒,货号CR01,96孔板:主要用于标准化人尿样中的分析物,与其他检测试剂盒配套使用。 4. 山羊抗15F2t-异前列烷,纯化的IgG,多抗,适用于免疫组化、酶联免疫、蛋白杂交,货号IS20,0.1 mg。 5. 葡糖醛酸糖苷酶样品预处理试剂盒(尿液预处理),货号GL85,40个样本:与ELISA试剂盒配套使用。人尿液中平均50%的异前列烷与葡糖
), cytomegalovirus (CMV), Epstein Barr Virus (EBV), herpes simplex virus (HSV) and other DNA-viruses in routinely formalin-fixed and paraffin-embedded tissues. ・ Detection of Human Papillomavirus in Cervical Carcinoma: Comparison of peroxidase
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