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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.054 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 大鼠S100钙结合蛋白A6(S100A6)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat S100A6(S100 Calcium Binding Protein A6) Microsample ELISA Kit |
| 别名 | S100-A6; 2A9; 5B10; CABP; CACY; PRA; MLN 4; Calcyclin; Growth factor-inducible protein 2A9; Prolactin receptor-associated protein |
| 货号 | ELK4261MS |
| 反应种属 | Rat |
| P05964 | P05964 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.054 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100A6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity;Infection immunity;Hematology;Dermatology; |

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文献和实验Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
S100 proteins are the largest subgroup of Ca2+ binding proteins with the EF-hand structural motif. A unique feature of this protein family is that individual members are localized in specific cellular compartments. For example, various S100
较少。研究者使用激光显微切割技术富集正常细胞和癌变的胰腺导管上皮细胞。从分离下来的细胞中分别提取蛋白,然后通过二维电泳进行分离。对二维电泳分离得到的蛋白进行银染以进行观察。当然,跑胶时确保使用相同的蛋白上样量非常关键。对比正常的和癌变的胰腺上皮细胞的蛋白图谱,发现其中九个蛋白点表达有差异。其中五个蛋白在肿瘤细胞中上调,而其中四个下调。其中一个在肿瘤细胞中表达增强的蛋白,被确定为钙结合蛋白,S100A6。 为了确定S100A6的过表达对胰腺癌的影响,我们使用了来自46个胰腺癌患者的174个重复点
Ci),双蒸水加至20μl。 (2)混合后,37℃水浴60min。 (3)加入1μl的0.5mol/L EDTA,90℃灭活5min。 5、观测 (1)取一定量的标记DNA稀释后在1.8%琼脂糖凝胶上点样,50V电泳约2h。 (2)电泳后的凝胶置滤纸上烘干,进行放射自显影。 (3)含32P标记DNA电泳干凝胶进行同位素液闪测定,计算放射活性。 6、结果判断: 正常活细胞DNA 电泳出现阶梯状(LADDER)条带;坏死细胞DNA电泳类似血抹片时的连续性条带。 三、酶联免疫吸附
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