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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.13 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人Ⅴ型胶原α2(COL5a2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human COL5a2(Collagen Type V Alpha 2) ELISA Kit |
| 别名 | COL5-A2; Collagen Alpha-2(V)chain; AB Collagen |
| 货号 | ELK3965 |
| 反应种属 | Human |
| P05997 | P05997 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.13 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5a2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL5a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL5a2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism; |

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文献和实验-nitrophenyl phosphate as substrate. Bone Gla protein(BGP) was measured by radioimmunoassay.Type I collagen,MMP-1,TIMP-1 mRNA was examined using semiquantitive reverse transcription-polymerase chain reaction(RT-PCR) analysis.Human osteosarcoma cell lines MG-63
,平均批间变异系数为11.4%;液相芯片与ELISA法检测结果的相关系数R2为0.88~0.99。 国外多家公司已推出了商品化的细胞因子液相芯片检测试剂盒,比如R&D 公司的Flurokine MAP系列、Linco Research公司的LINCOplex 试剂盒、BioSource公司的Antibody Bead Kit、Bio Rad公司的Bio Plex系列等,可用于来自人、小鼠、大鼠的样品,既有一次检测单种细胞因子的试剂盒,也有预先混合多种细胞因子抗体交联微球而一次检测多个指标
显微镜 仪器库 >> 影像系统 >>专业影像系统 试剂库 >> 免疫检测 >>ELISA检测试剂盒 抗体库 >> 抗体 抗体库 >> 第二抗体 >> 免疫组化二抗 抗体库 >> 试剂盒 >> 免疫组化试剂盒 仪器库 >> 分子生物实验仪器 >> 杂交/转印仪器 仪器库 >> 分子生物实验仪器 >> 杂交炉 试剂库 >>核酸检测 >> 原位杂交
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