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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.126 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人胰岛素样生长因子1受体(IGF1R)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human IGF1R(Insulin Like Growth Factor 1 Receptor) ELISA Kit |
| 别名 | CD221; IGF1-R; IGFIR; JTK13 |
| 货号 | ELK3396 |
| 反应种属 | Human |
| P08069 | P08069 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.126 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGF1R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGF1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGF1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGF1R in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Endocrinology;Cardiovascular biology;Hormone metabolism; |

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文献和实验Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas
The insulin-like growth factor II (IGFII) growth control pathway is comprised of three major components: IGFII ligand, insulin-like growth factor I receptor (IGFIR), and insulin-like growth factor II receptor (IGFIIR ). IGFIR and IGFIIR work
胰岛素受体底物(insulin receptor substrate,IRSs)
蛋白质,其上有多个(至少8个)可被受体激酶磷酸化的位点,磷酸化后可同多种效应物结合,包括:PI(3)K、Syp(一种磷酸酪氨酸磷酸酶)、Nck(一种连接蛋白)、GRB2(growth factor receptor-bound protein 2,一种通过SH2同磷酸化的酪氨酸结合的连接蛋白)。第二种是Shc(是通过cDNA克隆筛选到的编码SH结构域的基因的蛋白产物),也是一种连接蛋白。Shc的酪氨酸被磷酸化后能够同GRB2结合,然后激活Ras,触发细胞的增殖。第三种底物是IRS2。IRS2的酪氨酸被磷酸
受体酪氨酸激酶(receptor tyrosine kinase, RTKs)
growth factor, PDGF) 受体和巨噬细胞集落刺激生长因子(macrophage colony stimulating factor, M-CSF); ③胰岛素和胰岛素样生长因子-1 (insulin and insulin-like growth factor-1, IGF-1) 受体; ④神经生长因子(nerve growth factor, NGF) 受体; ⑤成纤维细胞生长因子(fibroblast growth factor, FGF) 受体
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