小鼠组织金属蛋白酶抑制因子3(TIMP3)酶联免疫吸附检测试
剂盒【Mouse TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.068 ng/mL
- 武汉
- ELK2521
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.068 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 小鼠组织金属蛋白酶抑制因子3(TIMP3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit |
| 别名 | SFD; TIMP Metallopeptidase Inhibitor 3; Metallopeptidase Inhibitor 3; Sorsby Fundus Dystrophy; Pseudoinflammatory |
| 货号 | ELK2521 |
| 反应种属 | Mouse |
| P39876 | P39876 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.068 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TIMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TIMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TIMP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity;Cardiovascular biology; |
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文献和实验多种细胞表达的基质金属蛋白酶。为探讨雌二醇（E2）对人成骨肉瘤MG-63细胞株和正常成人成骨细胞（hOB）膜型基质金属蛋白酶（MT1-MMP）的作用和绝经后骨质疏松症（OP）的作用机制。方法：Northern杂交、Western杂交和激光共聚焦显微系统免疫荧光法分别检测MT1-MMP mRNA和蛋白质表达。MMP-2活性用明胶酶谱和酶联免疫吸附测定检测。结果 首次观察到E2促进MG-63细胞MT1-MMPmRNA表达，并呈剂量依赖关系。E2促进MG-63细胞MT1-MMP蛋白质表达，并呈剂量
ArrayExpress http://www.ebi.ac.uk/arrayexpress Public collection of microarray gene expression data 17. Axeldb http://www.dkfz-heidelberg.de/abt0135/axeldb.htm Gene expression in Xenopus 18. BodyMap http://bodymap.ims.u-tokyo.ac.jp/ Human and mouse
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