相关产品推荐更多 >

大鼠突触蛋白Ⅰ(SYN1)酶联免疫吸附检测试剂盒【Rat SYN1(Synapsin I) ELISA Kit】
¥1960
马白介素12 p40(IL-12p40)酶联免疫吸附检测试剂盒【Horse IL-12p40(Interleukin 12 p40) ELISA Kit】
¥1960
人速激肽1(TAC1)酶联免疫吸附检测试剂盒【Human TAC1(Tachykinin, Precursor 1) ELISA Kit】
¥1820
人胃蛋白酶原A(PGA)酶联免疫吸附检测试剂盒【Human PGA(Pepsinogen A) ELISA Kit】
¥1820
小鼠Jagged 1蛋白(JAG1)酶联免疫吸附检测试剂盒【Mouse JAG1(Jagged 1 Protein) ELISA Kit】
¥1680
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.63-40 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.242 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 小鼠热休克蛋白40(HSP40)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse HSP40(Heat Shock Protein 40) ELISA Kit |
| 别名 | DnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1 |
| 货号 | ELK1496 |
| 反应种属 | Mouse |
| Q9QYJ3 | Q9QYJ3 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.242 ng/mL |
| 标准品 | 40 ng/mL |
| 检测范围 | 0.63-40 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSP40 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Developmental science; |

风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
Kit(10) 1313.00 75182 RNeasy Protect Maxi Kit(12) 4140.00 76104 RNAlater RNA Stabilizationeagent 682.00 * QIAGEN RNeasy Total RNA纯化系列(Affymetrix推荐!)采用QIAGEN著名的硅胶膜纯化柱技术,迅速特异地吸附样品裂解液中的RNA,无需酚氯仿抽提,不用乙醇沉淀或LiCl沉淀,也不用CsCl超离,只要20分钟(Mini Kit)到1小时(Maxi Kit
Kit一样,RNeasy Protect Kits采用QIAGEN著名的硅胶膜纯化柱技术,迅速特异地吸附样品裂解液中的RNA,无需酚氯仿抽提,不用乙醇沉淀或LiCl沉淀,也不用CsCl超离,只要洗脱即可得到纯的RNA。通常情况下RNeasy的纯化技术足以除去绝大部分的DNA,而无需额外进行DNaes处理,但是对于一些对痕量DNA非常敏感的实验,用RNase-Free DNase Set(QIAGEN cat.no. 79254)可以直接在纯化柱上消化DNA残留,在随后的洗涤步骤中除去DNase,最后
技术资料暂无技术资料 索取技术资料





